You might want to check out the wires leading to the docking loom inside the player. Assuming it worked before the disk upgrade, I am wondering if the drive tray could be cutting / grounding the signal wires leading to the loom.

Also, I assume there is no sign of cell phone mute or and other silencing option being set on the player.
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Paul Grzelak
200GB with 48MB RAM, Illuminated Buttons and Digital Outputs